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Our Plasmid DNA Extraction and Purification Kits

Purify high-quality plasmids (pDNA) from up to 200 mL bacterial culture with different plasmid types and culture medias

E.Z.N.A.® Plasmid DNA Mini Kit I

Purify up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes using the alkaline-lysis method and HiBind® Mini Column technology.

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pGEM plasmid was purified from 4 mL DH5α cultures harboring the plasmid and eluted in 50 µL volume using kits from Omega Bio-tek and Company Q according to manufacturer’s recommended protocols. Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system.

Learn more: E.Z.N.A.® Plasmid DNA Mini Kit I

  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Versatile – Spin and vacuum protocols available
  • High-quality – DNA is suitable for a variety of downstream applications

E.Z.N.A.® FastFilter Plasmid DNA Mini Kit

Purify high-quality plasmid DNA from up to 5 mL of culture following the alkaline-lysis method using a first-of-its-kind FastFilter Mini Column nested within a regular HiBind® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume.

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E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, culture medium, and input culture volume. The plasmid yield my vary based on these conditions. 

Learn more: E.Z.N.A.® FastFilter Plasmid DNA Mini Kit

  • Rapid – 9 Minute Processing time from a bacterial pellet
  • Unique – Lysate Clearance and DNA Binding in 1 step
  • Convenient – Lysate Clearance Column eliminates cellular debris pelleting and transfer step

E.Z.N.A.® Endo-free Plasmid DNA Mini Kit II

Purify up to 70 µg endotoxin-free plasmid DNA from 5-15 mL bacterial culture in less than 30 minutes using integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection.

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Quality and yield of plasmid DNA purified using the E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit. Plasmid DNA was isolated from 3 mL of three different bacterial cultures. DNA was eluted in 100 µL endotoxin-free elution buffer and quantitated using Thermo Fisher NanoDrop® 2000 and dynamic turbidity method.

Learn more: E.Z.N.A.® Endo-free Plasmid DNA Mini Kit II

  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Versatile – Spin and vacuum protocols available
  • High-quality – Endotoxins efficiently reduced (<1 EU/µg)

E.Z.N.A.® Plasmid DNA Mini Kit II

Purify 40-70 µg of high-quality DNA in less than 30 minutes using the increased DNA binding capacity of the HiBind® Mini Column II using culture volumes of up to 15 mL.

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The expected yield of 5 mL overnight cultures (LB medium) with the E.Z.N.A.® Plasmid Mini Kit II.

Learn more: E.Z.N.A.® Plasmid DNA Mini Kit II

  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

Mag-Bind® Endo-free Plasmid Mini Kit

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.

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pGEM plasmid DNA was purified from 1.5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Mini Kit, as well as Company P’s Kit, following manufacturers’ recommended protocol. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid preparation was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher compared to Company P’s Kit.

Learn more: Mag-Bind® Endo-free Plasmid Mini Kit

  • Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
  • High-yielding – Up to 20 μg plasmid DNA from 1.5 mL TB culture
  • Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg
  • Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8.

E.Z.N.A.® Plasmid DNA Midi Kit

Isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively.

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The expected yield of 50 mL overnight cultures (LB medium) with the E.Z.N.A.® Plasmid DNA Midi Kit.

Learn more: E.Z.N.A.® Plasmid DNA Midi Kit

  • Rapid – Purification of plasmid DNA in less than 60 minutes
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

E.Z.N.A.® FastFilter Plasmid DNA Midi Kit

Isolate up to 200 µg of high-quality plasmid DNA from 15-50 mL bacterial cultures in less than 40 minutes using Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis.

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Plasmid DNA was isolated from eight 30 mL bacterial cultures grown 16 hours in LB medium using the E.Z.N.A.® FastFilter Plasmid DNA Midi Kit and eluted in 300 µL elution buffer. Plasmid DNA (1% of total purified DNA) was analyzed on a 0.8% agarose gel. Lanes 1-8 represent plasmid DNA from 8 different bacterial cultures and Lane M represents the 1 kb DNA ladder.

Learn more: E.Z.N.A.® FastFilter Plasmid DNA Midi Kit

  • Rapid – Purification of plasmid DNA in less than 40 minutes using Lysate Clearance Filter Syringes instead of centrifugation
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit

Extract plasmid DNA from 15-50 mL of bacterial cultures in LB medium to produce up to 200 µg of high copy number plasmid DNA and process up to 100 mL of culture when working with low copy number plasmid. 

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pEGFP-N1 plasmid was purified from 50 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit following manufacturer’s recommended protocol. Plasmid DNA was eluted in 500 µL volume and quantified using Thermo Scientific’s NanoDrop™ 2000c system. The Endotoxin concentration was estimated based on a Limulus Amoebocyte Lysate (LAL) assay.

Learn more: E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit

  • Rapid – Purification of plasmid DNA in less than 45 minutes
  • Versatile – Spin and vacuum formats available
  • High-quality – Low-endotoxin plasmid DNA (< 1 EU/µg)

Mag-Bind® Endo-free Plasmid Midi Kit

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.

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Mag-Bind® Endo-free Plasmid Midi Kit is capable of handling diverse sample input conditions ranging from plasmid types, plasmid sizes, culture medium, and input culture volumes. The plasmid yield may vary based on these conditions.

Learn more: Mag-Bind® Endo-free Plasmid Midi Kit

  • Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
  • High-yielding – Plasmid DNA yield up to 200 μg
  • Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
  • Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
  • Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

E.Z.N.A.® Plasmid DNA Maxi Kit

Purify 600-1200 µg high copy number plasmid DNA or 50-300 µg low copy number plasmid DNA from 50-200 mL overnight culture.

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pGEM plasmid was purified from 250 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Maxi Kit following manufacturer’s recommended protocols. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.

Learn more: E.Z.N.A.® Plasmid DNA Maxi Kit

  • Rapid – Purification of plasmid DNA in < 60 minutes
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

E.Z.N.A.® Endo-Free Plasmid DNA Maxi KitI

Isolate plasmid DNA from 50-200 mL of bacterial cultures in LB medium to produce 0.6-1.2 mg of high copy number plasmid DNA or up to 500 mL of culture when processing low copy number plasmid.

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pEGFP-N1 plasmid was purified from 200 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit following manufacturer’s recommended protocol. Plasmid DNA was eluted in 2 mL volume and quantified using Thermo Scientific’s NanoDrop™ 2000c system. The Endotoxin concentration was estimated based on a Limulus Amoebocyte Lysate (LAL) assay.

Learn more: E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit

  • Rapid – Purification of plasmid DNA in less than 45 minutes
  • Versatile – Spin and vacuum formats available
  • High-quality – Low-endotoxin plasmid DNA (< 1 EU/µg)

E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit

Purify 50-200 mL of bacterial cultures in LB medium producing 0.6-1.2 mg of high copy number plasmid DNA using Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis.

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Total time in minutes required for plasmid DNA isolation from pelleting the bacterial cultures to eluting the plasmid using Omega Bio-tek E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit and comparable products from other competitors.

Learn more: E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit

  • Rapid – Purification of plasmid DNA in less than 40 minutes using Lysate Clearance Syringes to rapidly clear bacterial lysates post-lysis
  • Versatile – Spin and vacuum protocols available
  • High-quality – DNA is suitable for a variety of downstream applications